China CDC Primers and probes for detection 2019-nCoV (posted on 24 January 2020)
新型冠状病毒核酸检测引物和探针序列(Specific primers and probes for detection 2019 novel coronavirus)
Target 1(ORF1ab):
正向引物(F):CCCTGTGGGTTTTACACTTAA
反向引物(R):ACGATTGTGCATCAGCTGA
荧光探针(P):5′-FAM-CCGTCTGCGGTATGTGGAAAGGTTATGG-BHQ1-3′
Target 2(N):
正向引物(F):GGGGAACTTCTCCTGCTAGAAT
反向引物(R):CAGACATTTTGCTCTCAAGCTG
荧光探针(P):5′-FAM-TTGCTGCTGCTTGACAGATT-TAMRA-3′
CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel
Research Use Only 2019-Novel Coronavirus (2019-nCoV) Real-time RT-PCR Primers and Probes
Updated June 6, 2020
2019-nCoV_N1-F:GACCCCAAAATCAGCGAAAT
2019-nCoV_N1-R:TCTGGTTACTGCCAGTTGAATCTG
2019-nCoV_N1-P:FAM-ACCCCGCATTACGTTTGGTGGACC-BHQ1
2019-nCoV_N1-P:FAM-ACCCCGCAT/ZEN/TACGTTTGGTGGACC-3IABkFQ
2019-nCoV_N2-F:TTACAAACATTGGCCGCAAA
2019-nCoV_N2-R:GCGCGACATTCCGAAGAA
2019-nCoV_N2-P:FAM-ACAATTTGCCCCCAGCGCTTCAG-BHQ1
2019-nCoV_N2-P:FAM-ACAATTTGC/ZEN/CCCCAGCGCTTCAG-3IABkF
RNAse P-F:AGATTTGGACCTGCGAGCG
RNAse P-R:GAGCGGCTGTCTCCACAAGT
RNAse P-P:FAM–TTCTGACCTGAAGGCTCTGCGCG–BHQ-1
RNAse P-P:FAM-TTCTGACCT/ZEN/GAAGGCTCTGCGCG-3IABkFQ