China CDC Primers and probes for detection 2019-nCoV (posted on 24 January 2020)

新型冠状病毒核酸检测引物和探针序列(Specific primers and probes for detection 2019 novel coronavirus)

Target 1(ORF1ab):

正向引物(F):CCCTGTGGGTTTTACACTTAA

反向引物(R):ACGATTGTGCATCAGCTGA

荧光探针(P):5′-FAM-CCGTCTGCGGTATGTGGAAAGGTTATGG-BHQ1-3′

Target 2(N):

正向引物(F):GGGGAACTTCTCCTGCTAGAAT

反向引物(R):CAGACATTTTGCTCTCAAGCTG

荧光探针(P):5′-FAM-TTGCTGCTGCTTGACAGATT-TAMRA-3′

CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel

Research Use Only 2019-Novel Coronavirus (2019-nCoV) Real-time RT-PCR Primers and Probes

Updated June 6, 2020

2019-nCoV_N1-F:GACCCCAAAATCAGCGAAAT

2019-nCoV_N1-R:TCTGGTTACTGCCAGTTGAATCTG

2019-nCoV_N1-P:FAM-ACCCCGCATTACGTTTGGTGGACC-BHQ1

2019-nCoV_N1-P:FAM-ACCCCGCAT/ZEN/TACGTTTGGTGGACC-3IABkFQ

2019-nCoV_N2-F:TTACAAACATTGGCCGCAAA

2019-nCoV_N2-R:GCGCGACATTCCGAAGAA

2019-nCoV_N2-P:FAM-ACAATTTGCCCCCAGCGCTTCAG-BHQ1

2019-nCoV_N2-P:FAM-ACAATTTGC/ZEN/CCCCAGCGCTTCAG-3IABkF

RNAse P-F:AGATTTGGACCTGCGAGCG

RNAse P-R:GAGCGGCTGTCTCCACAAGT

RNAse P-P:FAM–TTCTGACCTGAAGGCTCTGCGCG–BHQ-1

RNAse P-P:FAM-TTCTGACCT/ZEN/GAAGGCTCTGCGCG-3IABkFQ